Questions about Microbes Photos for Webinar 7

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400x

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Marg Saul - What is this? 400x

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Marg Saul - What is happening with this nematode? Could it be shedding its skin? 400x

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Karen Tomkins -- Compost Photos from a local compost facility. They use a high end digestor machine that keeps it aerobic at all times but once it sits to cure they seem to have a lot of issues with anaerobic conditions.  Some of their batches are filled with actinobacteria, but some get this orange crust-like substance that will grow on the top surface instead. To the eye it looks like tiny orange lichen, but clearly that isn't it. I've attached a few photos. My guess is that it is an Oomycetes of some type and these are the spores? I couldn't find a lot of hyphae in the sample though.  What could they do other than introduce more oxygen to curb this? Any thoughts?  400x

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Karen Tomkins -- Compost Photos from a local compost facility. They use a high end digestor machine that keeps it aerobic at all times but once it sits to cure they seem to have a lot of issues with anaerobic conditions.  Some of their batches are filled with actinobacteria, but some get this orange crust-like substance that will grow on the top surface instead. To the eye it looks like tiny orange lichen, but clearly that isn't it. I've attached a few photos. My guess is that it is an Oomycetes of some type and these are the spores? I couldn't find a lot of hyphae in the sample though.  What could they do other than introduce more oxygen to curb this? Any thoughts?  400x

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Ruth Callard -- Here is a photo of a very uniform color tan thing with roundies in it.  400x

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Ruth Callard -- 400x

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Nathalie -- (400x) Photo 7 Mystery fungus (200x I think) I keep finding these, what I'd say are fungi - due to the filamentous way they grow, the dark color, septation. They're so distinctive in shape, always sprouting 4 ways, that I was surprised I haven't been able to find it online. On the second picture, you can see a hypha when it grows out.

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Nathalie -- (400x) Here's the grown out hypha. - But is this even a fungus, since it has this lumpy look to it?

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Pedro Carvalho -- 400x total MAG.  Testate Amoebae multiplying? Ciliate? I could not find the hairs around the body.

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Pedro Carvalho -- 400x total MAG. Bad fungal spores?

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Audrey Smith -- I was wondering what is happening with this fungal strand?

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Audrey Smith -- Wondering if this could be a developing microarthropod...

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400 total MAG

Fugal spore?

Root?

Protozoa spore/ egg?

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400x total MAG

Fungal spores?

The circles – bactéria?

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400x total MAG

9 – root?

10 – flagelate ?          

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400x total MAG

Fungal hyphae? Has color, has the septas. Diff in diameter is due to focus, or because is the direction of growing? I could not measure it right. I´m still struggling to identify the bacteria to do the measurement.  

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100x total MAG

root?

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400x total MAG

ciliate? Again, I could not find any hairs. Still have a lot to learn how to use the microscope but could that be that I have a bad calibration? Or is it a amoebae?

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400x total MAG

14- ???

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400x total MAG

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Ciliates: see attached picture

Can ciliates be tiny looking like gnats at 400X total mag? I've seen things that zoom in and out of the field of view doing curves and figure eights but don't look any larger than the smallest bacteria. Their movement is fast and perfectly smooth with no awkward motion as described for flagellates.  

Is the attached picture (400 total mag) of a dead ciliate (it never moved)? Is this their normal size? What is their size compared to a testate amoeba?

 

Flagellate or Bdeliovibrio:

At 400 total mag. the only thing I've seen with a flagella was zipping around and spinning (fast with no awkward motion). It had a long flagella (only one that I could see). It was small but was bigger than the bacteria. It was comma shaped. Can flagellates move like this? Can they be comma shaped? How do flagellates compare in size to a testate amoeba?

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